Journal: Biosensors

Article Title: Rapid Microfluidic Biosensor for Point-of-Care Determination of Rheumatoid Arthritis via Anti-Cyclic Citrullinated Peptide Antibody Detection

doi: 10.3390/bios14110545

Figure Lengend Snippet: Variation in OD intensity with anti-CCP Ab concentration for 2nd Ab-HRP reagents with different dilution factors. Error bars represent the range, calculated as the difference between the maximum and average values across three independent measurements.

Article Snippet: The anti-cyclic citrullinated peptide antibody (100 ng/mL = 30 IU/mL), sample diluent, and antibody diluent, as well as the 2nd antibody conjugate HRP (2nd Ab-HRP), were sourced from FineTest Inc. Additionally, 1% bovine serum albumin (BSA), diluted from a 10% stock solution was used as a blocking agent to reduce nonspecific binding, with phosphate-buffered saline (137 mM sodium chloride, 2.7 mM potassium chloride, 8.1 mM sodium hydrogen phosphate, 1.5 mM potassium dihydrogen phosphate), a wash buffer, and PBS containing 0.5% Tween 20 (PBST) used for rinsing steps.

Techniques: Concentration Assay

Journal: The Journal of Neuroscience

Article Title: Fas/Apo [Apoptosis]-1 and Associated Proteins in the Differentiating Cerebral Cortex: Induction of Caspase-Dependent Cell Death and Activation of NF-κB

doi: 10.1523/jneurosci.19-05-01754.1999

Figure Lengend Snippet: Figure 6. The expression of the Fas-associated proteins RIP (A), FADD (B), FLIP (C), and FasL (D) was examined by Western analysis of 50 mg of detergent-soluble protein obtained from P6 cerebral cortex obtained from four separate animals (lanes 1-4). Bands corresponding to RIP (74 kDa) and FasL (37 kDa) were detected in 10 mg of Jurkat cell lysates (a positive control) and in P6 cortex. FLIP expression in P6 cortex was confirmed by the appearance of a similar band just below the 66 kDa molecular weight marker, corresponding to the expected size of ;60 kDa, in protein extract from COS7 cell transfected with the full-length FLIP plasmid (positive control). In contrast, we did not detect any bands corresponding to the expected size for FADD in the cerebral cortex, although a band of the approximate size (24 kDa) was observed in Jurkat cell-derived protein (a positive control). Mkr, Biotinylated marker.

Article Snippet: Binding of the second, primary antibody was visualized by the appropriate secondary antibody, either a biotinylated donkey anti-rabbit antibody (Jackson ImmunoResearch, 1:500) or horse anti-mouse (Vector Laboratories, 1:1000) coupled to either streptavidin-FITC or rhodamineavidin.

Techniques: Expressing, Western Blot, Positive Control, Molecular Weight, Marker, Transfection, Plasmid Preparation, Derivative Assay